Five Parameter Logistic Curve

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(3 min 24 sec)

Quantitative analysis of samples using a Five Parameter Logistic (5PL) curve fit suitable for calculating concentrations from asymmetrical sigmoidal calibrators.

This analysis optionally includes a background correction step. If a blank group is included on your layout, the mean of the blank replicates is first subtracted from the raw data measurements (the corrected values are then used in the fit).

The standard data points (concentration vs. measurement) are plotted on semi-log axes and a 5PL is made through the points. The concentrations of the samples are determined from the fit with any specified dilution factors applied.

The %CV, Standard Deviation and Standard Error are calculated for each replicated sample.

Samples outside the range of the standards or the fit (greater than the upper asymptote or below than the lower asymptote) are highlighted in yellow.

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Standard Concentrations


Dilution Factors


Sample IDs


Run Notes